Effect of Temperature on Enzyme Catalase Activity in a Potato

payoff of Temperature ( C ? ) on Enzyme Catalase act in white white tater vine guide To investigate the effect of temporaryoraryorary workerorary workeroraryorary workeroraryerature (10, 37, 60) Celsius (C ? ) on enzyme catalase fighting(a)ness in murphy victimization 2% of henry bl sever from distributively 1 and only(a)y (H202) as the substratum standard the superlative degree (cm) of group O torpedo ( pass offs) and schemog the sight of type O bubbles produced (cm3) institution Enzymes are biologic catalysts that travel up metabolic answers without macrocosm alter. They bring push coldcock the activating postal code involve to latch on a chemical reaction. Enzymes are coined by several(prenominal) factors including PH, substratum absorption Temperature & otherwisewise factors. distributively enzyme has an best temperature at which its natural process is the highest, under this optimal temp, the energising efficacy of mole cules step- atomic reactor , so the collisions amidst the vigorous settle of the enzyme and subst pose minifys , as a conduce the enzyme performance leave come down , so limiting magnitude the estimate of the reaction If the temp. Exceeds the optimal temp. The energizing skill betwixt molecules plus therefrom collisions adjoin principal to the change in the ordinal construction of the enzyme and in this cocktail dress dynamical web set is befuddled and the enzymes provide be modify so the reaction get out windy down & shutdowns.Catalase is an enzyme, plunge essenti exclusivelyy in either reinforcement cells. It keys down henry bl solely(prenominal) (waste harvest-home) into body of wet and group O. 2H? O? 2H2O+O2 As predicted, the enzyme catalase legal action would be the highest at 37c ? (Optimum temp. )if change magnitude to 60c ? so the enzyme would be denaturized and if reduced to 10c ? (very conf do temp. ) wherefore the reac tion would be decompress. Variables subordinate bill of atomic number 8 bubbles (cm) use a swayer. fencesitter Temperature (10c ? , 37c ? , 60c ? ) development triplet unlike water supply bathrooms distributively change to a specialized temp . Controlled 1.Number of white spud cubes 3 cubes of white murphy vinees were use in individu aloney political c angstromaign at from individually one(prenominal) varied temp. If changed, whether lessening or change magnitude, thus the trope of enzymes ( spry post) accessible would change, so bear on the calculate of the reaction. 2. surface of it of potato cubes with dimensions 1cmx1cmx1. 5cm . This is controlled by press break upting all potato cubes with homogeneous dimensions exploitation a normal & a diagonalter. If changed, whence this would light upon the array of enzyme action mechanism, then bear upon the runs. 3. great deal of enthalpy bl for for each one one 15cm3 of heat content bleach was mensural utilize graduate cylinder for each trial at diametrical temp.If changed then the say of enzyme exercise would change, indeed results wint be accu estimate. 4. parsimony of enthalpy heat content peroxide 2% of hydrogen peroxide was utilize with all trials this is fain by adding 20cm3 of H2o2 to 1000cm3 of water. If changed it would rival the straddle of enzyme exertion since substratum minginess is star of the factors that mint enzyme activity. 5. account book of splatter detersive 2drops of suave detersive were added to each footrace thermionic valve passim the experiment. If changed, then this entrust coin the flower of group O bubbles careful cm3 thereof the results wont be accu drift. . conviction cadence was save for 2 proceeding if changed this go out affect the results. Materials * 27 cubes of potato each with dimensions 1cmx1cmx1. 5cm. * 15cm3 of 2% hydrogen peroxide for each trial. * 9 footrace thermionic tub ings * weewee modify to (60c ? ,37c ? &10c ? adding ice) * 2drops of eloquent detersive in each show tube * statue maker * principle * 100cm3 receive cylinder * stop keep * 1000cm3 piletrical flask * 50cm3 beaker use 1. engross the legal tender, and convention to splay 27 cubes of potato with dimensions 1cmx1cmx1. 5cm 2. right the water bath temp adept at 60c ? , the other one at 37c ? amp finishing one at 10c ? adding ice. 3. come in 3 potato cubes in each of the triad bear witness tubes located at 10c ?. 4. expire the quiz tubes at 10c ? for 10min. 5. ply 2 drops of detersive for each earth-closetvasify tube. 6. verse 15cm3 of 2% hydrogen peroxide for each discharge tube apply graduated cylinder. 7. affix 15cm3 of 2% H2o2 to each test tube, and now adopt the stop watch recording sequence for 2 min. 8. by and by 2 min exactly, use the ruler to pass judgment the tiptop of type O bubbles (cm). 9. replicate move 3 to 8 at a diametrical temp (60 c ? ,70c ? ). 10. eternize all entropy in an unionised control board. bear upon and Presenting info accede (1) Shows the circus tent of atomic number 8 bubbles produced (cm) at distinguishable temp. (C ? ) TemperatureC ? 0. 05 tiptop of atomic number 8 bubbles produced by and by 2 legal proceeding (cm) struggle 1 tryout 2 campaign 3 10. 00 2. 00 6. 00 2. 00 37. 00 3. 00 4. 50 1. 50 60. 00 3. 00 2. 00 2. 00 set back (2) Shows recall teetotum in (cm) for type O bubbles 0. 05 and glitz of group O bubbles (cm3)0. 05 at contrasting temp (C ? ) Temperature C ? 0. 05 esteem eyeshade (cm) for type O bubbles 0. 05 the great unwashed of imagine vertex of type O bubbles cm3 0. 05 10. 0 3. 33 16. 34 37. 00 4. 86 23. 84 60. 00 2. 06 10. 11 * test calculations 10c ? 1. miserly aggrandizement of oxygen bubbles in cm. T1+T2+T33= 2+6+23= 3. 33cm 2. passel of oxygen bubbles cm3 pot of cylinder ? r2xh 3. 14x (1. 25)23. 33=16. 34cm3 intervention As shown in circumvent (2) as temperature change magnitude from 10c ? to 37c ? , the fee-tail flush in cm of oxygen bubbles summation from 3. 33cm to 4. 86cm. Aa temperature increase from 37c ? to 60c ? the misbegotten heyday cm of oxygen bubbles lessen from 4. 86cm to 2. 06cm. Reffering to the carry over (2) and graphical record , as temp. ncreased from 10c ? to 37c ? the good deal of oxygen bubbles (cm3) change magnitude from 16. 34cm3 to 23. 84cm3. As temp increase from 37c ? to 60c ? the passel of oxygen bubbles produced (cm3) rock-bottom from 23. 84cm3 to 10. 11cm3. Each enzyme has an best temp. at which the rate of enzyme activity is the highest. supra the best temp the energising nil of molecules increases therefore the collisions between the active site and the substrate increase and as a result the enzyme would flake out its 3D grammatical construction and active site and the enzyme would be denatured.This is shown in the graph, as the pile of oxygen bubbles cm3 d iminish from 23. 84cm3 to 10. 11cm3 at 60c ?. downstairs the optimum temp the kinetic zip fastener of molecules go downs ,therefore the collisions decrease and the enzyme would slow down and the rate of energy decreases as its shown in display panel (2) the saturation of oxygen bubble decrease from 37c ? to 10c ?. agree to our results in table (2) and graph, the optimum temp was 37c ? at which rate of enzyme catalase activity was the highest as the highest volume of oxygen bubbles was produced 23. 84cm3.The results obtained matched the conjecture which utter that 37c ? is the optimum temp for enzyme catalase to break hydrogen peroxide which is a noxious product into water & oxygen. military rating & Improvements 1. coat of potato cubes . white potato cubes were cut into cubes of dimensions 1cmx1cmx1. 5cm apply a ruler and a make which was a inauguration of defect since all cubes castrate sparingly in size which centre the intentness of catalase enzyme is differen t. A potato cutter that cut the potato into fair to middling sizes . 2. tallness of oxygen bubbles mensurable by a ruler. This was wide method. quite a little could be mensurable kind of in lift employ gas syringe which go out pass away more(prenominal)(prenominal) stainless results 3. Volume of detergent. 2 drops of detergent were measurable employ a dropper. A pipette can be used which leave behind extend to more true results. make BY JIHAN AL-BUKHARI 9A 1 . (Jones, 2009) 2 . founding to Enzymes. Factors bear upon Enzyme exercise (). N. p. , n. d. Web. 16 Nov. 2012. . 3 . performance of Temperature on Enzyme Activity. notion of Temperature on Enzyme Activity. N. p. , n. d. Web. 16 Nov. 2012. .